| Place | Performance | Athlete | Team |
| 1 | 24.68 | Q: VAN DER MERWE - ZANTE | ATHLETICS GAUTENG NORTH |
| 2 | 24.87 | Q: VAN DYK - JENNA | ATHLETICS CENTRAL NORTHWEST |
| 3 | 25.01 | Q: MAKASI - LOLWETHU | CENTRAL GAUTENG ATHLETICS |
| 4 | 25.33 | q: VAN DER WESTHUYZEN - ELLENE | LIMPOPO ATHLETICS |
| 5 | 25.95 | -: TIVANE - SONIA | ATHLETICS MPUMALANGA |
| 6 | 26.15 | -: ALBERTS - BROOKLYN | ATHLETICS GAUTENG NORTH |
| 7 | 26.49 | -: MOTSHWANE - MOHAU | ATHLETICS CENTRAL NORTHWEST |
| 8 | 28.24 | -: MOSTERD - LIYA | NORTHWESTERN CAPE ATHLETICS |