| Place | Performance | Athlete | Team |
| 1 | 24.64 | Q: VERMAAK - HANNAH HOPE | CENTRAL GAUTENG ATHLETICS |
| 2 | 25.00 | Q: VAN STADEN - MICHE | WESTERN PROVINCE ATHLETICS |
| 3 | 25.38 | Q: CILLIERS - ZOë | ATHLETICS MPUMALANGA |
| 4 | 25.46 | q: PRETORIUS - LISA | ATHLETICS GAUTENG NORTH |
| 5 | 26.02 | -: PIENAAR - BUHLE | ATHLETICS GAUTENG NORTH |
| 6 | 26.11 | -: MGQIBELA - BUHLE | ATHLETICS GAUTENG NORTH |
| 7 | 29.05 | -: TINTO - LISAKHANYA | ATHLETICS TRANSKEI |