| Place | Performance | Athlete | Team |
| 1 | 24.22 | Q: BURGER - RUM | ATHLETICS FREE STATE |
| 2 | 25.38 | Q: BOTES - ZENIQUE | ATHLETICS GAUTENG NORTH |
| 3 | 25.71 | Q: FOUCHE - KEIRA | CENTRAL GAUTENG ATHLETICS |
| 4 | 25.81 | q: MAREE - KYLA (KYLA - MAE) | ATHLETICS CENTRAL NORTHWEST |
| 5 | 25.82 | -: SIBANYONI - KHANYA | ATHLETICS MPUMALANGA |
| 6 | 25.86 | -: MIENIE - MIEKE | LIMPOPO ATHLETICS |
| 7 | 26.21 | -: VERWEY - AMON | ATHLETICS FREE STATE |