| Place | Performance | Athlete | Team |
| 1 | 24.90 | Q: GUNTER - ISABELLA | ATHLETICS GAUTENG NORTH |
| 2 | 24.98 | Q: NDUBUEZE - OLUCHI ABELWE | CENTRAL GAUTENG ATHLETICS |
| 3 | 25.07 | Q: CORNELIUS - ASHLEY | BOLAND ATHLETICS |
| 4 | 25.79 | -: ORJI - ISOBEL | ATHLETICS FREE STATE |
| 5 | 26.27 | -: VILJOEN - LALU-JE | ATHLETICS CENTRAL NORTHWEST |
| 6 | 26.82 | -: GROENEWALD - MIKAYLIN | ATHLETICS FREE STATE |
| 7 | 26.92 | -: ROOS - DANELLE | LIMPOPO ATHLETICS |
| 8 | 27.98 | -: CHIPPS - RIHANA | ATHLETICS SOUTHWESTERN DISTRICTS |