| Place | Performance | Athlete | Team |
| 1 | 25.04 | Q: KONDE - FAITH | BOLAND ATHLETICS |
| 2 | 25.41 | Q: MAAKE - MOKGADI | ATHLETICS GAUTENG NORTH |
| 3 | 25.44 | Q: JORDAAN - JUAN | ATHLETICS VAAL TRIANGLE |
| 4 | 26.27 | -: MAKHANYA - FUNDISWA | ATHLETICS GAUTENG NORTH |
| 5 | 27.07 | -: NJEBO - SIPHESIHLE | CENTRAL GAUTENG ATHLETICS |
| 6 | 27.13 | -: BOATENG - CASSANDRA | ATHLETICS TRANSKEI |
| 7 | 27.33 | -: VAN DER WALT - DEONNE | ATHLETICS FREE STATE |
| 8 | 29.04 | -: STEYN - MEGAN | LIMPOPO ATHLETICS |