| Place | Performance | Athlete | Team |
| 1 | 24.84 | Q: KOOPMAN - TAYGEN | WESTERN PROVINCE ATHLETICS |
| 2 | 24.85 | Q: PRETORIUS - ANE | ATHLETICS GAUTENG NORTH |
| 3 | 25.02 | Q: REED - SARAH | KWAZULU-NATAL ATHLETICS |
| 4 | 25.26 | q: KAYSER - CHARNE | BOLAND ATHLETICS |
| 5 | 25.52 | q: BOTMA - CHENADE | ATHLETICS MPUMALANGA |
| 6 | 26.42 | -: SMITH - SUZELLE | ATHLETICS CENTRAL NORTHWEST |
| 7 | 26.45 | -: HILIZA - TUMELO | LIMPOPO ATHLETICS |
| 8 | 26.63 | -: MDAKA - IMITHA | CENTRAL GAUTENG ATHLETICS |