| Place | Performance | Athlete | Team |
| 1 | 25.12 | Q: VAN DER WESTHUYZEN - EMMA-LEE | LIMPOPO ATHLETICS |
| 2 | 25.27 | Q: VAN DER MERWE - DENE | ATHLETICS GAUTENG NORTH |
| 3 | 25.41 | Q: STICKLING - MILANA | BOLAND ATHLETICS |
| 4 | 25.61 | q: LOGGENBERG - NINA | ATHLETICS GAUTENG NORTH |
| 5 | 25.77 | q: OKOYE - PARIS | CENTRAL GAUTENG ATHLETICS |
| 6 | 26.96 | -: JANSEN VAN RENSBURG - ELOUISE | ATHLETICS VAAL TRIANGLE |
| 7 | 30.05 | -: MAKHABA - ZANELE | ATHLETICS TRANSKEI |