| Place | Performance | Athlete | Team |
| 1 | 42.87 | Q: MARTHINUSSEN - CUBIN | BOLAND ATHLETICS |
| 2 | 43.37 | Q: NIEMAN - MEGAN | ATHLETICS GAUTENG NORTH |
| 3 | 43.55 | q: KIRKPATRICK - JANELLE | ATHLETICS GAUTENG NORTH |
| 4 | 44.73 | -: DE KOCK - MIENKA | ATHLETICS FREE STATE |
| 5 | 45.79 | -: JULIAS - ABBIE | KWAZULU-NATAL ATHLETICS |
| 6 | 47.51 | -: RAUTENBACH - CARA | ATHLETICS FREE STATE |
| 7 | 47.93 | -: XABA - SIYANDA | ATHLETICS MPUMALANGA |