| Place | Performance | Athlete | Team |
| 1 | 42.69 | Q: GUNTER - ISABELLA | ATHLETICS GAUTENG NORTH |
| 2 | 43.31 | Q: BOUWER - KARLA | ATHLETICS GAUTENG NORTH |
| 3 | 44.73 | -: VAN DER ENDE - RUV | ATHLETICS GAUTENG NORTH |
| 4 | 45.80 | -: STOFFELS - CHLOE | BOLAND ATHLETICS |
| 5 | 48.18 | -: COETZEE - LIA | LIMPOPO ATHLETICS |
| 6 | 49.01 | -: SMITH - MEGAN | ATHLETICS CENTRAL NORTHWEST |