| Place | Performance | Athlete | Team |
| 1 | 42.61 | Q: LOGGENBERG - CHRISTI | ATHLETICS GAUTENG NORTH |
| 2 | 44.27 | Q: BOTHA - MIENKE | ATHLETICS FREE STATE |
| 3 | 44.41 | q: BOTES - ANIKA | ATHLETICS CENTRAL NORTHWEST |
| 4 | 46.33 | -: CASANOVES - MATHEIA | CENTRAL GAUTENG ATHLETICS |
| 5 | 46.56 | -: FOURIE - ZIONE | CENTRAL GAUTENG ATHLETICS |
| 6 | 46.81 | -: VAN DEN BERG - JAN | ATHLETICS CENTRAL NORTHWEST |