| Place | Performance | Athlete | Team |
| 1 | 39.04 | Q: AFRICA - MICAH | BOLAND ATHLETICS |
| 2 | 40.33 | -: MATHULOE - KATLEGO | ATHLETICS NORTHWEST NORTH |
| 3 | 40.40 | -: MASHIZHA - TAFADZWA | BOLAND ATHLETICS |
| 4 | 40.68 | -: MOLETSANE - BOKANG | ATHLETICS FREE STATE |
| 5 | 41.52 | -: NLHAPO - SIYA | ATHLETICS VAAL TRIANGLE |
| 6 | 42.32 | -: VORSTER - HAYDEN | CENTRAL GAUTENG ATHLETICS |
| 7 | 42.43 | -: BURGER - JANDRE | ATHLETICS MPUMALANGA |