| Place | Performance | Athlete | Team |
| 1 | 38.58 | Q: SCHOEMAN - RAY-JORDAN | ATHLETICS SOUTHWESTERN DISTRICTS |
| 2 | 38.61 | q: JANSE VAN RENSBURG - DIHANN | ATHLETICS GAUTENG NORTH |
| 3 | 39.99 | -: TSHESEBE - KAGISHO | ATHLETICS MPUMALANGA |
| 4 | 40.23 | -: VOSLOO - TIAAN | EASTERN PROVINCE ATHLETICS |
| 5 | 40.36 | -: MOTLAISANE - LERATO | ATHLETICS NORTHWEST NORTH |
| 6 | 42.42 | -: MKHABELA - TRUST | ATHLETICS MPUMALANGA |