| Place | Performance | Athlete | Team |
| 1 | 39.47 | Q: TIETUS - KEELAN | ATHLETICS CENTRAL NORTHWEST |
| 2 | 39.61 | q: BALOYI - MATIMBA | ATHLETICS GAUTENG NORTH |
| 3 | 40.66 | -: KGOSIENG - LEHLOHONOLO | ATHLETICS CENTRAL NORTHWEST |
| 4 | 41.53 | -: HOLLENBACH - CONNER | ATHLETICS CENTRAL NORTHWEST |
| 5 | 43.22 | -: MODISA - HLONI | ATHLETICS FREE STATE |