| Place | Performance | Athlete | Team |
| 1 | 37.86 | Q: BRINK - DANDRE | ATHLETICS GAUTENG NORTH |
| 2 | 38.63 | q: LESCH - MARCO | ATHLETICS SOUTHWESTERN DISTRICTS |
| 3 | 39.58 | q: HEDIN - SVEN | LIMPOPO ATHLETICS |
| 4 | 40.15 | -: KGAMANYANE - KARABELO | ATHLETICS NORTHWEST NORTH |
| 5 | 41.35 | -: DE BRUIN - HENDRIK (HENR) | CENTRAL GAUTENG ATHLETICS |
| 6 | 41.53 | -: DE WET - DIVAN | ATHLETICS CENTRAL NORTHWEST |