| Place | Performance | Athlete | Team |
| 1 | 0:59.16 | Q: VAN NIEKERK - HANNAH | ATHLETICS GAUTENG NORTH |
| 2 | 1:00.21 | Q: BLACK - ZANRI | EASTERN PROVINCE ATHLETICS |
| 3 | 1:01.06 | Q: DE KOCK - BARBARA | BOLAND ATHLETICS |
| 4 | 1:02.07 | q: SHIPALANA - PENELOPE | ATHLETICS GAUTENG NORTH |
| 5 | 1:04.68 | -: BEKKER - JESSY | CENTRAL GAUTENG ATHLETICS |