| Place | Performance | Athlete | Team |
| 1 | 1:01.83 | Q: DAVEL - MIENKE | ATHLETICS GAUTENG NORTH |
| 2 | 1:02.14 | Q: BASSON - KYLIE | BOLAND ATHLETICS |
| 3 | 1:03.92 | -: JENKINS - CAYLEN | CENTRAL GAUTENG ATHLETICS |
| 4 | 1:04.72 | -: COLLETT - JASMINE | ATHLETICS GAUTENG NORTH |
| 5 | 1:06.88 | -: PIENAAR - DANYELLE | ATHLETICS FREE STATE |
| 6 | 1:11.42 | -: BOTHA - CAYLIN | ATHLETICS NORTHWEST NORTH |