| Place | Performance | Athlete | Team |
| 1 | 1:00.10 | Q: WILSON - MIA | BOLAND ATHLETICS |
| 2 | 1:00.98 | Q: TEN CATE - AMY | WESTERN PROVINCE ATHLETICS |
| 3 | 1:01.35 | q: BURGER - ARETTE | ATHLETICS GAUTENG NORTH |
| 4 | 1:02.20 | q: ACHURCH - KAYLIN | ATHLETICS GAUTENG NORTH |
| 5 | 1:07.39 | -: NAUD - ANJA | CENTRAL GAUTENG ATHLETICS |
| 6 | 1:13.02 | -: EKSTEEN - SUMARI | ATHLETICS FREE STATE |