| Place | Performance | Athlete | Team |
| 1 | 1:00.94 | Q: RAMOKGOPA - TUMI | ATHLETICS GAUTENG NORTH |
| 2 | 1:02.28 | Q: VISSER - CARLI | ATHLETICS CENTRAL NORTHWEST |
| 3 | 1:02.68 | -: TRUTER - MARI | ATHLETICS FREE STATE |
| 4 | 1:04.15 | -: LOURENS - SIMON | ATHLETICS MPUMALANGA |
| 5 | 1:05.05 | -: OPPERMAN - KEIRA-LEE | CENTRAL GAUTENG ATHLETICS |