| Place | Performance | Athlete | Team |
| 1 | 0:51.41 | Q: MBATHA - NJABULO | ATHLETICS GAUTENG NORTH |
| 2 | 0:53.05 | Q: NDLOVU - MTHOKOZISI | ATHLETICS MPUMALANGA |
| 3 | 0:53.10 | q: PIENAAR - ALEX | CENTRAL GAUTENG ATHLETICS |
| 4 | 0:54.38 | q: BONTHUYS - ADRIAAN | ATHLETICS FREE STATE |
| 5 | 0:56.25 | -: HAASBROEK - JOHAN | LIMPOPO ATHLETICS |
| 6 | 1:01.86 | -: SCHOEMAN - ABELINES | BOLAND ATHLETICS |