| Place | Performance | Athlete | Team |
| 1 | 12.44 | Q: NIEMAN - MEGAN | ATHLETICS GAUTENG NORTH |
| 2 | 12.95 | q: FOURIE - KATE-LYNN | ATHLETICS CENTRAL NORTHWEST |
| 3 | 13.04 | q: DELL - KIARAH | ATHLETICS GAUTENG NORTH |
| 4 | 13.19 | q: STOFFELS - CHLOE | BOLAND ATHLETICS |
| 5 | 13.40 | -: GREYLING - LENE | ATHLETICS GAUTENG NORTH |
| 6 | 14.03 | -: CASANOVES - MATHEIA | CENTRAL GAUTENG ATHLETICS |