| Place | Performance | Athlete | Team |
| 1 | 0:50.18 | Q: MOLEFE - MOLEFE | CENTRAL GAUTENG ATHLETICS |
| 2 | 0:50.38 | Q: KOLOBE - LWANDELE | ATHLETICS MPUMALANGA |
| 3 | 0:51.80 | Q: BUTLER - MOHAMMED FUZAYL | CENTRAL GAUTENG ATHLETICS |
| 4 | 0:52.01 | -: TIETUS - KEELAN | ATHLETICS CENTRAL NORTHWEST |
| 5 | 0:53.68 | -: DE KOKER - JERNEILIO | ATHLETICS GAUTENG NORTH |
| 6 | 0:54.22 | -: SCHOEMAN - JOHAN | ATHLETICS SOUTHWESTERN DISTRICTS |