| Place | Performance | Athlete | Team |
| 1 | 0:56.08 | Q: LOGGENBERG - CHRISTI | ATHLETICS GAUTENG NORTH |
| 2 | 0:57.09 | Q: DE VILLIERS - LENE | ATHLETICS GAUTENG NORTH |
| 3 | 0:58.46 | Q: KETWANA - HLUMISA | ATHLETICS TRANSKEI |
| 4 | 0:58.60 | q: VAN DER ENDE - RUV | ATHLETICS GAUTENG NORTH |
| 5 | 0:59.47 | -: MASUKU - BOITUMELO | CENTRAL GAUTENG ATHLETICS |
| 6 | 0:59.76 | -: TALJAARD - NICKA | ATHLETICS VAAL TRIANGLE |
| 7 | 1:00.68 | -: BOTHA - CATHRINA MARIA | ATHLETICS GAUTENG NORTH |
| 8 | 1:01.89 | -: VAN DER MERWE - MARELIE | LIMPOPO ATHLETICS |