| Place | Performance | Athlete | Team |
| 1 | 0:56.87 | Q: JORDAAN - DENIKE | ATHLETICS CENTRAL NORTHWEST |
| 2 | 0:58.72 | Q: HOMANN - CELIA | ATHLETICS GAUTENG NORTH |
| 3 | 0:58.96 | Q: HEYNEKE - ANNERIKE | ATHLETICS GAUTENG NORTH |
| 4 | 0:59.08 | q: KAMELE - MBALI | CENTRAL GAUTENG ATHLETICS |
| 5 | 1:00.91 | -: BOTHA - MIENKE | ATHLETICS FREE STATE |
| 6 | 1:01.08 | -: ARONSON - TEEGAN | CENTRAL GAUTENG ATHLETICS |
| 7 | 1:01.52 | -: VAN DER WALT - DEONNE | ATHLETICS FREE STATE |
| 8 | 1:02.63 | -: NEETHLING - DAN | ATHLETICS FREE STATE |