| Place | Performance | Athlete | Team |
| 1 | 45.75 | Q: LOEDOLFF - CHIRSTE | ATHLETICS GAUTENG NORTH |
| 2 | 45.04 | Q: NEL - CHRIS-MARI | BOLAND ATHLETICS |
| 3 | 44.84 | Q: WATSON - JUDY | ATHLETICS FREE STATE |
| 4 | 43.49 | Q: OLIVIER - JAMOR | CENTRAL GAUTENG ATHLETICS |
| 5 | 43.08 | Q: ENGELBRECHT - ELZE | ATHLETICS NORTHWEST NORTH |
| 6 | 41.83 | Q: ODENDAAL - IZA-MARIZE | CENTRAL GAUTENG ATHLETICS |
| 7 | 41.24 | Q: BRINK - DANEL | ATHLETICS VAAL TRIANGLE |
| 8 | 40.98 | Q: BREYTENBACH - CARA | ATHLETICS GAUTENG NORTH |
| 9 | 40.55 | Q: COETZEE - MIEKE | ATHLETICS SOUTHWESTERN DISTRICTS |
| 10 | 39.53 | Q: ODENDAAL - NICOLA | ATHLETICS GAUTENG NORTH |
| 11 | 38.64 | Q: JANSE VAN RENSBURG - MERLE | ATHLETICS GAUTENG NORTH |
| 12 | 38.15 | Q: STEYNBERG - DONELI | ATHLETICS CENTRAL NORTHWEST |
| 13 | 37.77 | -: RAUTENBACH - LISA | ATHLETICS FREE STATE |
| 14 | 37.62 | -: UECKERMANN - MILA | WESTERN PROVINCE ATHLETICS |
| 15 | 36.77 | -: NEL - ASHLYNN | ATHLETICS GAUTENG NORTH |
| 16 | 36.24 | -: MAMPE - REATLEGILE | ATHLETICS CENTRAL NORTHWEST |
| 17 | 35.13 | -: TALJAARD - CARLI | ATHLETICS FREE STATE |
| 18 | 35.09 | -: NEL - ZAN | ATHLETICS MPUMALANGA |
| 19 | 34.59 | -: PIENAAR - KEIRA | CENTRAL GAUTENG ATHLETICS |
| 20 | 34.18 | -: LOUW - LYNN-MARI | ATHLETICS FREE STATE |
| 21 | 33.78 | -: PRETORIUS - ROZL | ATHLETICS CENTRAL NORTHWEST |
| 22 | 31.89 | -: CHALMERS - ALISON | WESTERN PROVINCE ATHLETICS |
| 23 | 30.39 | -: LEMMER - ANE | ATHLETICS FREE STATE |
| 24 | 28.37 | -: ROTHMANN - MARIKE | KWAZULU-NATAL ATHLETICS |