| Place | Performance | Athlete | Team |
| 1 | 12.15 | Q: REED - SARAH | KWAZULU-NATAL ATHLETICS |
| 2 | 12.21 | Q: KOOPMAN - TAYGEN | WESTERN PROVINCE ATHLETICS |
| 3 | 12.36 | q: ORJI - ISOBEL | ATHLETICS FREE STATE |
| 4 | 12.47 | -: FOURIE - KATE-LYNN | ATHLETICS CENTRAL NORTHWEST |
| 5 | 12.48 | -: PRETORIUS - ANE | ATHLETICS GAUTENG NORTH |
| 6 | 12.88 | -: BREFO - ANGELA | ATHLETICS MPUMALANGA |
| 7 | 12.92 | -: HILIZA - TUMELO | LIMPOPO ATHLETICS |
| 8 | 12.99 | -: KOTZE - KATELYN | ATHLETICS MPUMALANGA |