| Place | Performance | Athlete | Team |
| 1 | 0:46.50 | Q: MARTIN - WIAAN (IND) | BOLAND ATHLETICS |
| 2 | 0:46.78 | Q: VAN DERWALT - TJAART | EASTERN PROVINCE ATHLETICS |
| 3 | 0:47.06 | Q: OOSTHUIZEN - RUAN | ATHLETICS GAUTENG NORTH |
| 4 | 0:48.56 | q: SNYDERS - JJ | ATHLETICS FREE STATE |
| 5 | 0:50.63 | -: RAMATHE - KGOTSO | CENTRAL GAUTENG ATHLETICS |