| Place | Performance | Athlete | Team |
| 1 | 0:45.38 | Q: PILLAY - LYTHE | CENTRAL GAUTENG ATHLETICS |
| 2 | 0:46.74 | Q: MTHIMKULU - MTHI | ATHLETICS FREE STATE |
| 3 | 0:47.99 | Q: MATLALA - THATO | ATHLETICS GAUTENG NORTH |
| 4 | 0:48.83 | q: MOSIKILI - MOFANA | ATHLETICS VAAL TRIANGLE |
| 5 | 0:48.91 | -: GARDEE - RASHEED | CENTRAL GAUTENG ATHLETICS |
| 6 | 0:49.15 | -: MBATHA - XOLANI | KWAZULU-NATAL ATHLETICS |
| 7 | 0:50.04 | -: MOTLOBA - KAMOGELO | ATHLETICS NORTHWEST NORTH |