| Place | Performance | Athlete | Team |
| 1 | 0:48.39 | Q: LABUSCHAGNE - JANES | ATHLETICS CENTRAL NORTHWEST |
| 2 | 0:48.54 | Q: MAWELA - SOLETHU | ATHLETICS NORTHWEST NORTH |
| 3 | 0:48.68 | Q: FRASER - WJ | ATHLETICS MPUMALANGA |
| 4 | 0:48.81 | q: VISSER - ANDR | ATHLETICS CENTRAL NORTHWEST |
| 5 | 0:48.98 | q: PIENAAR - JACQUES | ATHLETICS CENTRAL NORTHWEST |
| 6 | 0:50.84 | -: MAAMOGAWA - THUTO | CENTRAL GAUTENG ATHLETICS |
| 7 | 0:51.03 | -: MACHETE - KGOTSO | ATHLETICS MPUMALANGA |
| 8 | 0:52.08 | -: MIENIES - STUART | ATHLETICS GRIQUALAND WEST |