| Place | Performance | Athlete | Team |
| 1 | 0:48.71 | Q: KATOO - BRYAN | EASTERN PROVINCE ATHLETICS |
| 2 | 0:49.19 | Q: DU PLESSIS - JUAN | BORDER ATHLETICS |
| 3 | 0:49.25 | Q: VAN ZYL - ROUX | CENTRAL GAUTENG ATHLETICS |
| 4 | 0:49.34 | q: GROBLER - HENDRIK | ATHLETICS GAUTENG NORTH |
| 5 | 0:52.42 | -: MEHLO - LIOR | ATHLETICS TRANSKEI |
| 6 | 1:07.20 | -: SEILANE - TSATSI DANIEL | ATHLETICS CENTRAL NORTHWEST |