| Place | Performance | Athlete | Team |
| 1 | 0:49.47 | Q: BOIKHUTSO - TSHIAMO | KWAZULU-NATAL ATHLETICS |
| 2 | 0:49.59 | Q: BRIEL - DANIEL | ATHLETICS CENTRAL NORTHWEST |
| 3 | 0:50.05 | Q: SWANEPOEL - NIEL | ATHLETICS GAUTENG NORTH |
| 4 | 0:50.48 | -: PRINS - MARCO | ATHLETICS GAUTENG NORTH |
| 5 | 0:50.75 | -: NOMANDLA - ZWELAKHE | ATHLETICS FREE STATE |
| 6 | 0:53.92 | -: LUBALA - CHUMANI | ATHLETICS TRANSKEI |