| Place | Performance | Athlete | Team |
| 1 | 0:55.80 | Q: SCHEEPERS - COLENE | ATHLETICS GAUTENG NORTH |
| 2 | 0:56.91 | Q: VERMEULEN - CHANE | CENTRAL GAUTENG ATHLETICS |
| 3 | 0:57.14 | Q: THOMSEN - MICHAELA | ATHLETICS CENTRAL NORTHWEST |
| 4 | 0:57.20 | q: SCHEEPERS - NINA | BOLAND ATHLETICS |
| 5 | 0:57.48 | q: VAN HEERDEN - JESSICA | ATHLETICS CENTRAL NORTHWEST |
| 6 | 0:58.86 | -: VAN DER LINDE - MARLENE | ATHLETICS GAUTENG NORTH |
| 7 | 0:59.41 | -: THALE - KAMOGELO | ATHLETICS NORTHWEST NORTH |