| Place | Performance | Athlete | Team |
| 1 | 55.40 | Q: ERASMUS - AIDAN | ATHLETICS GAUTENG NORTH |
| 2 | 55.29 | Q: STEVENS - TIAAN | ATHLETICS GAUTENG NORTH |
| 3 | 49.75 | Q: VAN RENSBURG - WALTER | ATHLETICS SOUTHWESTERN DISTRICTS |
| 4 | 48.76 | Q: SENEKAL - ERIK | ATHLETICS GAUTENG NORTH |
| 5 | 48.51 | Q: DU TOIT - JADE | LIMPOPO ATHLETICS |
| 6 | 48.01 | Q: HELBERG - JOHANNES | LIMPOPO ATHLETICS |
| 7 | 47.80 | Q: NEL - JOHACHIM | ATHLETICS NORTHWEST NORTH |
| 8 | 47.00 | Q: NEL - HANRO | ATHLETICS CENTRAL NORTHWEST |
| 9 | 46.51 | Q: MONYAKE - SHAUN | ATHLETICS GAUTENG NORTH |
| 10 | 46.26 | Q: MULLER - JUHIN | ATHLETICS FREE STATE |
| 11 | 46.21 | Q: PRINSLOO - WILLEM | ATHLETICS CENTRAL NORTHWEST |
| 12 | 46.07 | Q: MARITZ - NICKUS | ATHLETICS VAAL TRIANGLE |
| 13 | 44.66 | -: SWANEPOEL - CHRIS | ATHLETICS GAUTENG NORTH |
| 14 | 44.41 | -: GOEDHALS - HEINRICH | ATHLETICS SOUTHWESTERN DISTRICTS |
| 15 | 43.19 | -: BRITZ - TIAAN | ATHLETICS VAAL TRIANGLE |
| 16 | 42.85 | -: REYNEKE - REAGAN | KWAZULU-NATAL ATHLETICS |
| 17 | 42.46 | -: NEL - KEANU | CENTRAL GAUTENG ATHLETICS |
| 18 | 41.81 | -: DROTSKIE - LUHAN | LIMPOPO ATHLETICS |
| 19 | 41.65 | -: VAN DER LINDE - JC | ATHLETICS FREE STATE |
| 20 | 39.20 | -: VAN DER ZWAARD - CONRAD | ATHLETICS FREE STATE |