| Place | Performance | Athlete | Team |
| 1 | 0:49.51 | Q: DIKGALE - LESIBE | ATHLETICS GAUTENG NORTH |
| 2 | 0:49.55 | Q: COETZEE - JAEGER | BOLAND ATHLETICS |
| 3 | 0:49.91 | Q: MHLONGO - PRINCE | ATHLETICS FREE STATE |
| 4 | 0:49.99 | Q: CHAMBERS - JOHAN | CENTRAL GAUTENG ATHLETICS |
| 5 | 0:50.69 | q: NDLOVU - SIMPHIWE | ATHLETICS GAUTENG NORTH |
| 6 | 0:51.80 | -: CLOETE - LOYISO | NORTHWESTERN CAPE ATHLETICS |