| Place | Performance | Athlete | Team |
| 1 | 0:47.42 | Q: ROMIJN - KRYN | ATHLETICS MPUMALANGA |
| 2 | 0:49.65 | Q: PIENAAR - ALEX | CENTRAL GAUTENG ATHLETICS |
| 3 | 0:49.96 | Q: MDAKA - TINTSWALO | ATHLETICS FREE STATE |
| 4 | 0:50.46 | Q: KOENA - OARABILE | ATHLETICS CENTRAL NORTHWEST |
| 5 | 0:51.39 | -: MAUNGO - KGOSIETSILE | ATHLETICS NORTHWEST NORTH |
| 6 | 0:53.80 | -: LUNAM - KWEZI | ATHLETICS TRANSKEI |