| Place | Performance | Athlete | Team |
| 1 | 0:49.58 | Q: MARUMA - LEEMA | ATHLETICS GAUTENG NORTH |
| 2 | 0:49.97 | Q: MYBURGH - STIAAN | CENTRAL GAUTENG ATHLETICS |
| 3 | 0:50.23 | Q: KADMIEL - LOTIKA | LIMPOPO ATHLETICS |
| 4 | 0:50.30 | Q: MYBURG - BEN | ATHLETICS GAUTENG NORTH |
| 5 | 0:50.74 | q: MKHWANAZI - TSHIDISO | ATHLETICS GAUTENG NORTH |
| 6 | 0:51.24 | q: LABUSCHAGNE - GERHARD | ATHLETICS VAAL TRIANGLE |
| 7 | 0:51.55 | -: HUMAN - HERNU | ATHLETICS CENTRAL NORTHWEST |
| 8 | 0:54.82 | -: MODISANA - TSHEPISO | ATHLETICS TRANSKEI |