| Place | Performance | Athlete | Team |
| 1 | 0:57.22 | Q: ACHURCH - KAYLIN | ATHLETICS GAUTENG NORTH |
| 2 | 0:57.37 | Q: ESTERHUYSE - VEN | CENTRAL GAUTENG ATHLETICS |
| 3 | 0:57.42 | q: VISSER - CARLI | ATHLETICS CENTRAL NORTHWEST |
| 4 | 0:58.32 | -: NEL - EULA (IND) | BOLAND ATHLETICS |
| 5 | 0:58.89 | -: PRETORIUS - LISA | ATHLETICS GAUTENG NORTH |
| 6 | 1:00.25 | -: PHALANE - ROSINA | LIMPOPO ATHLETICS |
| 7 | 1:02.28 | -: EKSTEEN - SUMARI | ATHLETICS FREE STATE |
| 8 | 1:02.30 | -: MOTSHWANE - MOHAU | ATHLETICS CENTRAL NORTHWEST |