| Place | Performance | Athlete | Team |
| 1 | 0:56.75 | Q: BURGER - ARETTE | ATHLETICS GAUTENG NORTH |
| 2 | 0:56.80 | Q: DAVEL - MIENKE | ATHLETICS GAUTENG NORTH |
| 3 | 0:57.36 | q: FOURIE - ASTRID | ATHLETICS VAAL TRIANGLE |
| 4 | 0:57.79 | -: REINSTEIN - NOA | KWAZULU-NATAL ATHLETICS |
| 5 | 0:59.48 | -: SWIEGERS - ELRISA | ATHLETICS VAAL TRIANGLE |
| 6 | 1:00.37 | -: CROUSE - ANE | ATHLETICS FREE STATE |
| 7 | 1:01.42 | -: MADLAKUVANA - SISPHO | ATHLETICS GAUTENG NORTH |
| 8 | 1:05.14 | -: FELDMAN - TEHILAH | LIMPOPO ATHLETICS |