| Place | Performance | Athlete | Team |
| 1 | 0:56.15 | Q: VAN DER MERWE - ZANTE | ATHLETICS GAUTENG NORTH |
| 2 | 0:56.97 | Q: STRONG - DAVIDA | CENTRAL GAUTENG ATHLETICS |
| 3 | 0:57.48 | -: VESTER - NECKE | ATHLETICS FREE STATE |
| 4 | 1:00.13 | -: DE VILLIERS - JOHANKA | ATHLETICS VAAL TRIANGLE |
| 5 | 1:00.61 | -: DE WET - CHARNE | LIMPOPO ATHLETICS |
| 6 | 1:01.86 | -: BLIGNAUT - MIENKE | ATHLETICS CENTRAL NORTHWEST |
| 7 | 1:03.27 | -: DU PLESSIS - MAKAYLA | LIMPOPO ATHLETICS |
| 8 | 1:04.96 | -: MTSHIYO - ASEKHONA | ATHLETICS TRANSKEI |