| Place | Performance | Athlete | Team |
| 1 | 0:52.29 | Q: MCASLIN - DEAN | ATHLETICS CENTRAL NORTHWEST |
| 2 | 0:53.65 | Q: SCHOEMAN - JOHAN | ATHLETICS SOUTHWESTERN DISTRICTS |
| 3 | 0:54.02 | Q: NXUMALO - UNATHI | KWAZULU-NATAL ATHLETICS |
| 4 | 0:54.25 | -: NLHAPO - SIYA | ATHLETICS VAAL TRIANGLE |
| 5 | 0:54.29 | -: MAHLANGU - NTANDO | ATHLETICS MPUMALANGA |
| 6 | 0:54.29 | -: MOENG - AMOGELANG | ATHLETICS NORTHWEST NORTH |