| Place | Performance | Athlete | Team |
| 1 | 0:51.34 | Q: MOLEFE - MOLEFE | CENTRAL GAUTENG ATHLETICS |
| 2 | 0:51.93 | Q: TIETUS - KEELAN | ATHLETICS CENTRAL NORTHWEST |
| 3 | 0:52.13 | Q: MEYER - LEZARIO | BOLAND ATHLETICS |
| 4 | 0:52.93 | q: NYONI - ONEIL | ATHLETICS GAUTENG NORTH |
| 5 | 0:53.31 | -: NAIDOO - NOAH | ATHLETICS GAUTENG NORTH |
| 6 | 0:53.68 | -: BALOYI - MATIMBA | ATHLETICS GAUTENG NORTH |
| 7 | 0:53.91 | -: NAGEL - NASHWIN | LIMPOPO ATHLETICS |
| 8 | 0:55.02 | -: TANSI - KGOTSO | ATHLETICS FREE STATE |