| Place | Performance | Athlete | Team |
| 1 | 0:59.13 | Q: HOMANN - CELIA | ATHLETICS GAUTENG NORTH |
| 2 | 0:59.29 | Q: KAMELE - MBALI | CENTRAL GAUTENG ATHLETICS |
| 3 | 0:59.96 | Q: HEYNEKE - ANNERIKE | ATHLETICS GAUTENG NORTH |
| 4 | 1:00.84 | q: BOTHA - CATHRINA MARIA | ATHLETICS GAUTENG NORTH |
| 5 | 1:01.34 | q: NEETHLING - DAN | ATHLETICS FREE STATE |
| 6 | 1:01.34 | -: KLEINSMITH - AMERIZE | CENTRAL GAUTENG ATHLETICS |