| Place | Performance | Athlete | Team |
| 1 | 0:57.96 | Q: JORDAAN - DENIKE | ATHLETICS CENTRAL NORTHWEST |
| 2 | 0:59.00 | Q: KETWANA - HLUMISA | ATHLETICS TRANSKEI |
| 3 | 0:59.71 | Q: MASUKU - BOITUMELO | CENTRAL GAUTENG ATHLETICS |
| 4 | 0:59.77 | q: BOTHA - MIENKE | ATHLETICS FREE STATE |
| 5 | 1:01.48 | -: SWANEPOEL - LEE-ANNE | ATHLETICS NORTHWEST NORTH |
| 6 | 1:02.67 | -: KEKANA - MICHELLE | ATHLETICS GAUTENG NORTH |