| Place | Performance | Athlete | Team |
| 1 | 0:59.49 | Q: DE VILLIERS - LENE | ATHLETICS GAUTENG NORTH |
| 2 | 1:00.34 | Q: VAN DER WALT - DEONNE | ATHLETICS FREE STATE |
| 3 | 1:00.47 | Q: TALJAARD - NICKA | ATHLETICS VAAL TRIANGLE |
| 4 | 1:01.31 | q: VAN DER MERWE - MARELIE | LIMPOPO ATHLETICS |
| 5 | 1:02.62 | -: TANNOUS - AVA | ATHLETICS CENTRAL NORTHWEST |
| 6 | 1:02.97 | -: ROOME - MICHELA | CENTRAL GAUTENG ATHLETICS |