| Place | Performance | Athlete | Team |
| 1 | 0:58.22 | Q: LOGGENBERG - CHRISTI | ATHLETICS GAUTENG NORTH |
| 2 | 1:00.44 | Q: VAN DER ENDE - RUV | ATHLETICS GAUTENG NORTH |
| 3 | 1:01.17 | Q: ARONSON - TEEGAN | CENTRAL GAUTENG ATHLETICS |
| 4 | 1:01.93 | -: HEYMANS - LYNN | ATHLETICS VAAL TRIANGLE |
| 5 | 1:02.07 | -: SWIEGERS - ANIKA | ATHLETICS VAAL TRIANGLE |
| 6 | 1:03.89 | -: LIYATI - MICHELLE | ATHLETICS TRANSKEI |
| 7 | 1:04.12 | -: VILJOEN - LALU-JE | ATHLETICS CENTRAL NORTHWEST |