| Place | Performance | Athlete | Team |
| 1 | 11.83 | Q: NOLTE - DANIELLE | ATHLETICS GAUTENG NORTH |
| 2 | 11.87 | Q: NEL - RHODE | ATHLETICS GAUTENG NORTH |
| 3 | 12.09 | Q: ROTHMAN - AMIEL | ATHLETICS CENTRAL NORTHWEST |
| 4 | 12.25 | -: NDLELA - MELODY | KWAZULU-NATAL ATHLETICS |
| 5 | 12.29 | -: NKWINIKA - NEO FAIRHOPE | CENTRAL GAUTENG ATHLETICS |
| 6 | 12.49 | -: SCHOONBEE - BIANCA | EASTERN PROVINCE ATHLETICS |
| 7 | 12.56 | -: EDWARDS - CHAN | CENTRAL GAUTENG ATHLETICS |
| 8 | 12.68 | -: MBOMBO - ALIYAH | WESTERN PROVINCE ATHLETICS |