| Place | Performance | Athlete | Team |
| 1 | 11.70 | Q: BOTJE - KAILI | CENTRAL GAUTENG ATHLETICS |
| 2 | 12.09 | Q: VAN NIEKERK - TENINKE | ATHLETICS MPUMALANGA |
| 3 | 12.32 | -: PETERSEN - ANGIE (IND) | BOLAND ATHLETICS |
| 4 | 12.39 | -: VAN DEN BERG - MENIKE | ATHLETICS GAUTENG NORTH |
| 5 | 12.70 | -: OORMEYER - ALYSSA | EASTERN PROVINCE ATHLETICS |
| 6 | 12.84 | -: MARIMO - SHAUNA | BORDER ATHLETICS |
| 7 | 13.36 | -: LETSOALO - MAITE` | LIMPOPO ATHLETICS |
| 8 | 13.49 | -: CLOETE - RIONA | NORTHWESTERN CAPE ATHLETICS |