| Place | Performance | Athlete | Team |
| 1 | 12.00 | Q: BURGER - RUM | ATHLETICS FREE STATE |
| 2 | 12.14 | Q: VAN OUDTSHOORN - KAYLIE | CENTRAL GAUTENG ATHLETICS |
| 3 | 12.23 | Q: CILLIERS - ZO | ATHLETICS MPUMALANGA |
| 4 | 12.46 | q: VAN ROOYEN - KATHLEEN | ATHLETICS GAUTENG NORTH |
| 5 | 12.55 | q: SMOOK - ZELMARIE | ATHLETICS FREE STATE |
| 6 | 12.58 | -: FOUCHE - KEIRA | CENTRAL GAUTENG ATHLETICS |
| 7 | 12.78 | -: MOTLATLE - AMOGELANG | ATHLETICS GAUTENG NORTH |
| 8 | 12.83 | -: BILLING - AMONE | ATHLETICS SOUTHWESTERN DISTRICTS |