| Place | Performance | Athlete | Team |
| 1 | 11.92 | Q: VERMAAK - HANNAH HOPE | CENTRAL GAUTENG ATHLETICS |
| 2 | 11.95 | Q: ROCHA - SELENA | ATHLETICS GAUTENG NORTH |
| 3 | 12.12 | Q: BOTES - ZENIQUE | ATHLETICS GAUTENG NORTH |
| 4 | 12.58 | -: TIVANE - SONIA | ATHLETICS MPUMALANGA |
| 5 | 12.58 | -: NORMAN - ASHLYNN | CENTRAL GAUTENG ATHLETICS |
| 6 | 12.80 | -: BESTER - MIKAYLA | LIMPOPO ATHLETICS |
| 7 | 13.23 | -: DINTOE - KHUMO | LIMPOPO ATHLETICS |
| 8 | 13.66 | -: MOSTERD - LIYA | NORTHWESTERN CAPE ATHLETICS |