| Place | Performance | Athlete | Team |
| 1 | 12.25 | Q: STREET - CHELSEA | EASTERN PROVINCE ATHLETICS |
| 2 | 12.33 | Q: VAN STADEN - MICHE | WESTERN PROVINCE ATHLETICS |
| 3 | 12.41 | Q: MAKASI - LOLWETHU | CENTRAL GAUTENG ATHLETICS |
| 4 | 12.43 | q: ESTEVES - MELISSA | ATHLETICS MPUMALANGA |
| 5 | 12.53 | q: MAREE - KYLA (KYLA - MAE) | ATHLETICS CENTRAL NORTHWEST |
| 6 | 12.72 | -: ALBERTS - BROOKLYN | ATHLETICS GAUTENG NORTH |
| 7 | 12.82 | -: VAN WYK - ANIKA | ATHLETICS FREE STATE |