| Place | Performance | Athlete | Team |
| 1 | 12.22 | Q: ORJI - ISOBEL | ATHLETICS FREE STATE |
| 2 | 12.29 | Q: VAN ZYL - LIZ | ATHLETICS CENTRAL NORTHWEST |
| 3 | 12.51 | Q: PRETORIUS - ANE | ATHLETICS GAUTENG NORTH |
| 4 | 12.81 | Q: HILIZA - TUMELO | LIMPOPO ATHLETICS |
| 5 | 12.84 | q: BREFO - ANGELA | ATHLETICS MPUMALANGA |
| 6 | 13.13 | -: XABA - SIYANDA | ATHLETICS MPUMALANGA |